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Then, the cells were incubated on ice in a 1 : 10 PBS mobile phase solution containing metaphosphoric acid 5% for 30 min

Furthermore, our enzyme kinetic assays showed that although 6-hydroxy-FAD-reconstituted hFSP1 exhibited lower affinities ( K m ) for NADH and CoQ 1 than FAD-reconstituted hFSP1, the NADH oxidation activity ( k cat ) of 6-hydroxy-FAD-reconstituted hFSP1 was higher than that of FAD-reconstituted hFSP1 (Supplementary Table 3)

Given the lack of comparative studies on the interaction between chelators and efflux transporters, our aim was to establish a comprehensive experimental model to evaluate and compare the effects of the three major MDR ABC transporters on the toxicity of metal chelators

2-page printable handout Prepared by Danielle Alcala-Glazier Hormone & Cellular Wellness Wellness Science Series Sermorelin & Growth Hormone Natural GH signaling, sleep, body composition & anti-aging